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ACTA Pharmaceutica Sciencia 2024 , Vol 62 , Num 1
Monitoring the charge variant profile of antibody-tomaymycin conjugates by icIEF method
Ayat ABBOOD 1
1 Department of Medicinal Chemistry and Quality Control, Faculty of Pharmacy, Tishreen University, Lattakia, Syria
DOI : 10.23893/1307-2080.APS6215 This study aimed to evaluate the charge variant profile of antibody-tomaymycin conjugates using an imaged capillary isoelectric focusing method (icIEF). Two unconjugated antibodies were examined, along with two conjugation methods involving cleavable and non-cleavable linkers. The cleavable linker contained one amine group. mAb-1 demonstrated greater homogeneity with a single main peak at a pI of 8.5, whereas mAb-2 exhibited two main peaks at pI values of 8.95 and 9.00. Conjugation with tomaymycin molecules resulted in an increased charge heterogeneity of both cleavable and non-cleavable conjugates. The non-cleavable conjugates displayed a higher number of additional acidic variants (pI range: 7.4 to 8.4 for mAb-1 and 8.2 to 8.9 for mAb-2) compared to the cleavable conjugates (pI range: 7.8 to 8.4 for mAb-1 and 8.4 to 8.9 for mAb-2). The linker nature had a lesser influence on the charge heterogeneity of the antibody-tomaymycin conjugates compared to the nature of the unconjugated antibody. Keywords : antibody, charge isoforms, conjugates, linker

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