ACTA Pharmaceutica Sciencia 2009 , Vol 51 , Num 2
IMMOBILIZATION AND PROPERTIES OF BACILLUS CIRCULANS LEVANSUCRASE
HEBA A EL-REFAİ, AHMED F ABDEL-FATTAH, FATEN A MOSTAFA
Chemistry of Natural and Microbial Products Dept., National Research Center, Dokki, Cairo, Egypt Levansucrase from a local isolate of Bacillus circulans was immobilized on wool adopting the physical adsorption technique. The relative activity of the immobilized enzyme was 55.20 % per g carrier and the specific activity (calculated on bound protein basis) was 78.12% of the free (soluble) enzyme. Compared to the free enzyme, the immobilized form revealed a higher optimal reaction temperature, low activation energy, higher Km (Michaelis constant) and lowers Vmax (maximal reaction rate). The same enzyme preparation exhibited thermal stability and resistance to chemical denaturation. Keywords : B CIRCULANS, LEVAN, IMMOBILIZATION, LEVANSUCRASE, ENZYME PROPERTIES

Istanbul Medipol University