ACTA Pharmaceutica Sciencia 2007 , Vol 49 , Num 3
PREPARATION AND IN VITRO EVALUATION OF BROMHEXINE-HCL LOADED POLY(L-LACTIC ACID) MICROSPHERES
DEMET ŞENSOY, SEVGİ GÜNGÖR, NAZAN BERGİSADİ
Department of Pharmaceutical Technology, Faculty of Pharmacy, Istanbul University, Istanbul, Turkey The aim of this study was to prepare and characterize Bromhexine-HCl (BRMX) loaded Poly(L-lactic acid) (PLA) microspheres by solvent evaporation technique using different polymer:drug ratios (1:1, 2:1 and 5:1). The surface characteristics, drug content, particle size and distribution, production yield were examined and encapsulation efficiency, drug-polymer interaction and in vitro drug release were also determined. SEM results demonstrated that all of the BRMX loaded PLA microspheres have regular shaped smooth surface properties. Actual drug contents were 25.77% ± 0.69, 16.54% ± 0.92 and 8.17% ± 0.41 for the microspheres with polymer:drug ratios 1:1, 2:1, and 5:1 respectively. BRMX-loaded PLA microspheres, ranged between 1.53 ± 0.01 – 2.12 ± 0.02 µm with the production yield 66.49% ± 1.65– 44.74% ± 1.89. Encapsulation efficiency of drug loaded microspheres was established between 34.27% ± 4.30 – 23.67% ± 2.21. The DSC thermograms showed that BRMX has no interaction with the polymer used. In vitro release studies indicated that all BRMX-loaded PLA microspheres followed sustained release pattern for 72 h. Keywords : BROMHEXINE HCL; MICROSPHERE; PLA; SUSTAINED RELEASE; SOLVENT EVAPORATION METHOD

Istanbul Medipol University