| Acceptance rate | 46% |
|---|---|
| Time to first decision | 6 months* |
| Time to decision with review | 50 days* |
*Approximate number of days
**The days mentioned above are averages and do not indicate exact durations. The process may vary for each article.
ACTA Pharmaceutica Sciencia
2009 , Vol 51 , Num 2
IMMOBILIZATION AND PROPERTIES OF BACILLUS CIRCULANS LEVANSUCRASE
Chemistry of Natural and Microbial Products Dept., National Research Center, Dokki, Cairo, Egypt
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Levansucrase from a local isolate of Bacillus circulans was immobilized on wool adopting the physical adsorption technique. The relative activity of the immobilized enzyme was 55.20 % per g carrier and the specific activity (calculated on bound protein basis) was 78.12% of the free (soluble) enzyme. Compared to the free enzyme, the immobilized form revealed a higher optimal reaction temperature, low activation energy, higher Km (Michaelis constant) and lowers Vmax (maximal reaction rate). The same enzyme preparation exhibited thermal stability and resistance to chemical denaturation.
Keywords :
B CIRCULANS, LEVAN, IMMOBILIZATION, LEVANSUCRASE, ENZYME PROPERTIES