| Acceptance rate | 46% |
|---|---|
| Time to first decision | 6 months* |
| Time to decision with review | 50 days* |
*Approximate number of days
**The days mentioned above are averages and do not indicate exact durations. The process may vary for each article.
ACTA Pharmaceutica Sciencia
2022 , Vol 60 , Num 3
Efficacy of Liquid-Liquid Extraction and Protein Precipitation Methods in Serum Sample Preparation for Quantification of Fexofenadine in Human Serum
1 Pharmaceutical Sciences Research Division, BCSIR Laboratories, Dhaka, Bangladesh Council of Scientific and Industrial Research (BCSIR), Dhaka-1205, Bangladesh2 Department of Applied Chemistry and Chemical Engineering, University of Dhaka, Dhaka-1205, Bangladesh
DOI : 10.23893/1307-2080.APS.6018 Viewed : 9571 - Downloaded : 4301 Biological samples contain many intrinsic and extrinsic compounds in different concentrations which makes it very challenging to analyze it and cannot normally be injected directly into the analyzing system without sample preparation. Two different sample extraction procedures liquid-liquid extraction and protein precipitation have been employed in this study to quantify fexofenadine in human serum and the recovery rates have been compared. Protein precipitation by methanol has an advantage over liquid-liquid extraction with recovery rates of more than 90% but has limitations due to rise of column back pressure. Due to better recovery rate and quick sample preparation technique the protein precipitation method has been chosen for extraction of drug from serum sample. The developed HPLC method was validated and found to be accurate, precise and specific within the linearity range of 0.8- 4.0 µg/mL. Lower limits of detection and quantification were established as 0.6 and 0.8 µg/mL respectively. Keywords : Liquid-liquid extraction, protein precipitation, bioanalysis, sensitivity