| Acceptance rate | 46% |
|---|---|
| Time to first decision | 6 months* |
| Time to decision with review | 50 days* |
*Approximate number of days
**The days mentioned above are averages and do not indicate exact durations. The process may vary for each article.
ACTA Pharmaceutica Sciencia
2009 , Vol 51 , Num 2
SHIFTING PRODUCTION OF RIFAMYCIN-SV BY MUTANT OF AMYCOLATOPSIS MEDITERRANEI TOLERANT TO BENZYL ALCOHOL
National Research Center, Che. of Natural and Microbial Products Dept., Dokki-Cairo-EGYPT
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Benzyl alcohol is a toxic hydrophobic compound for the microorganisms but using at specific concentration, 0.25%, a mutant of A. mediterranei-CBS-42575 was selected which has the maximum metabolism shifting of rifamycin-B from 1460 into 95(mg l-1) into rifamycin-SV upto 4.896 (g l-1). At the optimum fermentation differentiation between A. mediterranei-CBS- 42575 and its benzyl alcohol mutant in kinetic analysis was established, the resulted values for the growth yield coefficient YX/S was decreased from 0.4663 for the parent strain into 0.311 and 0.3675 (g cells g glucose-1 l-1) for the mutant in the shake flasks and the fermentor respectively. Also Monod growth saturation coefficient (Ks) recorded values, 1.01 for the parent strain while 0.95 and 1.57 (g S l-1) for the mutant in the shake flasks and the fermentor respectively. Also, YP/X was 0.373 for the parent strain and increased upto 1.0756 and 0.98 (g rif.-SV g cells-1) for the mutant in the shake flasks and the fermentor respectively. Also, the maximum of yield coefficients of refamycin-SV, YP/S, were obtained, 0.2523 and 0.3144 (g rif.-SV g glucose-1) for the mutant in the shake flasks and fermentor respectively.
Keywords :
RIFAMYCIN-SV, AMYCOLATOPSIS MEDITERRANEI, BENZYL ALCOHOL, HYDROPHOBIC COMPOUNDS, FERMENTATION, KINETIC ANALYSES