| Acceptance rate | 46% |
|---|---|
| Time to first decision | 6 months* |
| Time to decision with review | 50 days* |
*Approximate number of days
**The days mentioned above are averages and do not indicate exact durations. The process may vary for each article.
ACTA Pharmaceutica Sciencia
2011 , Vol 53 , Num 1
TOXICITY ASSESSMENT OF A Β-GALACTOSIDE-BINDING LECTIN (GALECTIN-1) FROM BULLFROG RANA CATESBEIANA UNFERTILIZED EGGS
1Laboratory of Glycobiology and Marine Biochemistry, Department of Genome System Science, Graduate School of Nano Biosciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama
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The present study was undertaken to investigate the toxicity of β-galactoside-binding lectin (galectin-1) using the brine shrimp lethality bioassay technique. Galectin-1 was purified from the American bullfrog Rana catesbeiana unfertilized eggs by conventional affinity chromatography method. The molecular mass of the galectin-1 was determined to be 15 kDa by SDS-PAGE under non-reducing and reducing conditions, respectively. Galectin-1 affects significantly the mortality rate (56.66%) of brine shrimp (Artemia salina L.) lethality bioassay. The results exhibited that galectin-1 was found to be moderate toxic and the mortality rate of brine shrimp nauplii was increased with the increase in concentration of galectin- 1. These findings suggest that the galectin-1 obtained from amphibian may have anticancer and antitumor activity and would be useful to clinical microbiology to play a significant role in human therapy.
Keywords :
GALECTIN-1, RANA CATESBEIANA, BRINE SHRIMP, TOXICITY, MORTALITY