ACTA Pharmaceutica Sciencia 2011 , Vol 53 , Num 1
TOXICITY ASSESSMENT OF A Β-GALACTOSIDE-BINDING LECTIN (GALECTIN-1) FROM BULLFROG RANA CATESBEIANA UNFERTILIZED EGGS
SARKAR M A KAWSAR, SHEİKH AFTABUDDİN, GOLAM MOSTAFA, HİDETARO YASUMİTSU, YASUHİRO OZEKİ
1Laboratory of Glycobiology and Marine Biochemistry, Department of Genome System Science, Graduate School of Nano Biosciences, Yokohama City University, 22-2 Seto, Kanazawa-ku, Yokohama The present study was undertaken to investigate the toxicity of β-galactoside-binding lectin (galectin-1) using the brine shrimp lethality bioassay technique. Galectin-1 was purified from the American bullfrog Rana catesbeiana unfertilized eggs by conventional affinity chromatography method. The molecular mass of the galectin-1 was determined to be 15 kDa by SDS-PAGE under non-reducing and reducing conditions, respectively. Galectin-1 affects significantly the mortality rate (56.66%) of brine shrimp (Artemia salina L.) lethality bioassay. The results exhibited that galectin-1 was found to be moderate toxic and the mortality rate of brine shrimp nauplii was increased with the increase in concentration of galectin- 1. These findings suggest that the galectin-1 obtained from amphibian may have anticancer and antitumor activity and would be useful to clinical microbiology to play a significant role in human therapy. Keywords : GALECTIN-1, RANA CATESBEIANA, BRINE SHRIMP, TOXICITY, MORTALITY

Istanbul Medipol University