ACTA Pharmaceutica Sciencia 2009 , Vol 51 , Num 2
SHIFTING PRODUCTION OF RIFAMYCIN-SV BY MUTANT OF AMYCOLATOPSIS MEDITERRANEI TOLERANT TO BENZYL ALCOHOL
KAMAL Y I EL-SHAHED
National Research Center, Che. of Natural and Microbial Products Dept., Dokki-Cairo-EGYPT Benzyl alcohol is a toxic hydrophobic compound for the microorganisms but using at specific concentration, 0.25%, a mutant of A. mediterranei-CBS-42575 was selected which has the maximum metabolism shifting of rifamycin-B from 1460 into 95(mg l-1) into rifamycin-SV upto 4.896 (g l-1). At the optimum fermentation differentiation between A. mediterranei-CBS- 42575 and its benzyl alcohol mutant in kinetic analysis was established, the resulted values for the growth yield coefficient YX/S was decreased from 0.4663 for the parent strain into 0.311 and 0.3675 (g cells g glucose-1 l-1) for the mutant in the shake flasks and the fermentor respectively. Also Monod growth saturation coefficient (Ks) recorded values, 1.01 for the parent strain while 0.95 and 1.57 (g S l-1) for the mutant in the shake flasks and the fermentor respectively. Also, YP/X was 0.373 for the parent strain and increased upto 1.0756 and 0.98 (g rif.-SV g cells-1) for the mutant in the shake flasks and the fermentor respectively. Also, the maximum of yield coefficients of refamycin-SV, YP/S, were obtained, 0.2523 and 0.3144 (g rif.-SV g glucose-1) for the mutant in the shake flasks and fermentor respectively. Keywords : RIFAMYCIN-SV, AMYCOLATOPSIS MEDITERRANEI, BENZYL ALCOHOL, HYDROPHOBIC COMPOUNDS, FERMENTATION, KINETIC ANALYSES

Istanbul Medipol University