ACTA Pharmaceutica Sciencia 2010 , Vol 52 , Num 3
SIMULTANEOUS ESTIMATION OF RISPERIDONE, OLANZAPINE AND QUETIAPINE AND THEIR DEGRADATION PRODUCTS BY HPLC
DEEPA SHARMA, KONA S SRİNİVAS, PALLAVİ GUPTA, DHANANJAY P DWİVEDİ, HARİSH DUREJA, MANJU NAGPAL, PANKAJ RAKHA, SURİNDER GOYAL
Department of Pharmacy, Rajendra Institute of Technology and Sciences, 4th Miles Stone, Hisar Road, Sirsa, India A rapid, specific reversed phase HPLC method has been developed for simultaneous determination of risperidone, olanzapine and quetiapine. Drugs were subjected to stress conditions such as acidic, alkaline and oxidative hydrolysis. Chromatographic separation of these pure drugs was carried out on Luna C18 (2504.6, 56m) with a 50:50 (v:v) mixture of 20mM ammonium acetate and acetonitrile as mobile phase. The flow rate was 1.0 mL min-1 and the analysis was monitored at 235 nm by UV detection. The system and method precision was found to be less than 1%. The assay results were linear from 35 to 65 μg mL=1 for risperidone (R2 > 0.991), olanzapine (R2 > 0.992) and quetiapine (R2 > 0.999). Method validated showed it to be specific, precise, robust and linear over the range of analysis. Separation was complete within 10 min. Degradation studies revealed that degradation products do not interfere with the determination of drugs. Keywords : RISPERIDONE, OLANZAPINE, QUETIAPINE, HPLC, FORCE DEGRADATION

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