ACTA Pharmaceutica Sciencia
2005 , Vol 47 , Num 3
ROLE OF SOME FERMENTATION PARAMETERS AFFECTING LIPASE PRODUCTION BY FUSARIUM SOLANI
Natural and Microbial Products Chemistry Department, National Research Center (NRC), Ddokki, Cairo, Egypt
The lipase production by F. Solani was inwestigated on using shaking flask and laboratory stirred fermenter. The results indicated that the best yield 380 U/ml (specific enzyme activity 9.04 U/mg protein) was obtained by using Erlenmeyer flask 250 ml containing 50 ml of culture with a medium composition g%: Sucrose 2, corn oil 0.2 ml, MgSO4.7H2O 0.05, KCI 0.05, KH2PO4 0.1, (NH4)2SO4 0.23, at pH 6.2 using citrate phosphate buffer and inoculum size 6 ml/50 ml medium. However, on using a bench top fermenter 5 L, the yield was increased by about one and half fold (520U/ml) under optimized fermentation conditions.
Keywords :
LIPASE PRODUCTION, F SOLANI