ACTA Pharmaceutica Sciencia 2011 , Vol 53 , Num 2
EFFECT OF MURRAYA KOENIGII PRETREATMENT ON THE TRANSPORT OF BUSPIRONE ACROSS RAT INTESTINE
SHRAVAN KUMAR YAMSANİ, ADUKONDALU DEVANDLA, VAMSHİ VİSHNU YAMSANİ, RAMESH GANNU, CHİNNA REDDY PALEM, SHİVA KUMAR RAVULA, BHARGAVİ ATHUKURİ MADHUSUDAN RAO YAMSANİ, SARANGAPANİ MANDA
Centre for Biopharmaceutics and Pharmacokinetics, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, A.P. India To investigate the effect of Murraya koenigii fruit (aqueous extract) pretreatment on transport of buspirone, a CYP3A4 substrate across the rat intestine. The transport of buspirone across rat intestine (duodenum, jejunum and ileum) was studied by using non-everted sac method. The rats were pretreated with Murraya koenigii fruit (aqueous extract) for 7 days. The rats were sacrificed by using anesthetic ether, the intestinal segments were isolated and used for the studies. The probe drug (buspirone) solution was placed in the isolated intestinal sac. Samples were collected at preset time points and replaced with fresh buffer. The drug content in the samples was estimated using high performance liquid chromatography method. Control experiments were also performed. The results revealed that there was a significant (p<0.05) difference compared to control, in the transport of buspirone from the intestinal sacs which were pretreated with M. koenigii fruit (aqueous extract). It suggests that both M. koenigii aqueous extract might be acting by inhibiting the transporters and enzymes which are responsible for transport/metabolism of buspirone. From the results it can be concluded that M. koenigii fruit aqueous extract might be acting by inhibiting CYP3A4 enzymes as buspirone is extensively metabolized by CYP3A4. Further studies are recommended to prove their effects in human beings. Keywords : CYTOCHROME P-450 3A, NONEVERTED SAC, MURRAYA KONEGII

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