ACTA Pharmaceutica Sciencia 2020 , Vol 58 , Num 2
Microbiological and Histological Characteristics of Interactions Between Carvacrol and Fluconazole in a Systemic Candidiasis Animal Model
Zeinab Hakim 1 Fahimeh Alizadeh 1 Esmaeel Panahi Kokhdan 2 Alireza Khodavandi 3
1 Department of Microbiology, Yasooj Branch, Islamic Azad University, Yasooj, Iran
2 Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
3 Department of Biology, Gachsaran Branch, Islamic Azad University, Gachsaran, Iran
DOI : 10.23893/1307-2080.APS.05811 The objectives of the study were to explore the effect of carvacrol on Candida tropicalis in vitro and in animal model, alone and in combination with fluconazole. The activity of carvacrol alone and in combination with fluconazole were tested on C. tropicalis using the CLSI reference method and in murine models. Carvacrol in combination with fluconazole showed satisfactory activity, with FICI mainly in the range of 0.375-1.00. Microbiologically, treatment with carvacrol alone in combination with fluconazole decreased the fungal load of kidneys of infected animal at negligible level of the number of colony counts of C. tropicalis. Histologically, in treated animals, no Candida organisms were found in the kidney tissues; this was in contrast to control groups in which many yeasts mixed with hyphae were observed. Carvacrol alone in combination with fluconazole as an alternative approach could be considered as the extensive opportunities for the treatment and prevention of systemic candidiasis. Keywords : Animal models; Candida tropicalis; Candidiasis; in vitro

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